Download Citation on ResearchGate | Actinobacillus seminis isolated from cattle | The organism commonly referred to as Actinobacillus. Actinobacillus seminis is a well known cause of epididymitis in Australian rams ( Baynes and Simmons, ) and has also been reported as associated with. The main causative agents of ovine infectious epididymitis are Brucella ovis, Actinobacillus seminis, and Histophilus somni [1–3].

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These profiles provide the first data, to semminis knowledge, regarding subcellular fractions of A. The Scientific World Journal. The length and width of the tail of epididymis were measured with caliper. Actinobacillus seminis is a well known cause of epididymitis in Australian rams Baynes and Simmons, and has also been reported as associated with metritis, polyarthritis Higgins et alposthitis and mastitis Watt et al Evers cautioned against a actinobbacillus and cull program based on A. In ampullae of the vas deferens, interstitial and intraluminal inflammatory infiltrate Figure 8 dmild to severe diffuse mononuclear interstitial infiltrate, multifocal neutrophilic intraepithelial infiltrate, and glandular hyperplasia were observed.

The polypeptide with a molecular mass of 75 kDa identified in the fractions and had an apparently specific immunogenic reaction: A sensitive silver stain for detection of lipopolysaccharides in polyacrylamide gels.

Protein concentration was determined by the method of Bradford Differences were considered statistically different when. The owner of a mob of mixed age first cross ewes reported that weeks prior to lambing due to start mid Septemberseven ewes aborted or produced stillborn premature lambs.


Actinobacillus seminis (Baynes and Simmons) Sneath and Stevens ATCC &r

There is a single report of A. To avoid cross-contamination between semen samples from different actinobacillus in each group, we used a sterile, disposable plastic inside the artificial vagina, connected directly to collection tube. Figure 1B shows the corresponding Western-blot results, which were different for each fraction, showing high antigenic reactivity by the 3 subcellular fractions when tested with the antiserum against whole cells of A.

Throughout the experimental period, there was no change of scrotal circumference in both experimental groups Figure 1 a. The rams were evaluated once prior to inoculation and every seven days of postinoculation dpiduring six weeks, totaling seven evaluations by the experimental group.

The analyses were performed with the GraphPad InStat program, version 3.


Ovine brucellosis caused by B. To visualize the A.

The left actibobacillus had reduced size and consistency, and in one ram atrophy of the left testis associated with diffuse fibrosis of the tunica vaginalis was observed Figure 5 c.

Multifocal fibrous adhesions between the tunica albuginea and the tunica vaginalis. These findings unequivocally demonstrate that the bacterium genealogically corresponded to A.

The Scientific World Journal

Table I Bacterial strains and isolates used in this study. Immunoblotting of membrane microvesicles To detect the kDa protein in membrane microvesicles of A. The length and width of the left epididymides tails of rams H.


Body of left epididymis is increased in size and focal area in cut surface with yellowish viscous material. However, the measurements of the left tail of the epididymides i. The latter differentiates A. An invertebrate coagulation system activated by endotoxin: All reagents were from Sigma Chemical Company, St. Traduit par Docteur Serge Messier. Al-Katib and Dennis, Subclinical cases have been known to persist for up to 4 years.

To determine if the kDa protein was species-specific for A. Support Center Support Center. Al-Katib and Dennis recommended continual surveillance against the introduction of infected rams into a semminis flock and identifying and removing infected animals. The isolate was further investigated by API32E biochemical testing which didn’t result in a confirmed identification.

Actinobacillus seminis – Wikipedia

The losses are due to lower fertility rate, reduction in the number of birth, and early culling of breeders [ 23 ]. The recovery of bacteria was possible in semen and urine samples and tissues in both experimental groups.

Actinobacillus seminis is a gram-negative bacterium of the Pasteurellaceae family that is involved in ovine epididymitis. It was confirmed that A. Looking for a actunobacillus specific to this species, we determined the protein profile of subcellular fractions of A.

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